Panasonic scientists set a low temperature record without harmful CFCs. Now, Panasonic mechanically refrigerated cryogenic freezers achieves stable
long-term preservation of cells and tissues. Ideally suited for ultra
low and cryogenic storage in laboratories, long-term preservation and
storage of blood, specimens and components, and in testing of various
types. New VIP® PLUS technology maximizes storage capacity over
conventionally insulated models. Whatever your preservation needs are, Panasonic provides the right equipment to meet your exact requirements. Panasonic preservation systems employ advanced technology to insure a high
precision temperature environment.
-150°C Cryogenic Temperature Freezer Ensures Stable Cell and Tissue preservation
Cryopreservation allows for storage of biological materials at ultra
low temperatures, while minimizing the level of freezing damage to
cells. An important factor to consider when preserving cells or tissue
at cryogenic temperatures is to prevent amorphous ice crystals from
recrystallizing within and outside the cells. These ice crystals
increase the degree of freezing damage to cells. When ice crystals
become amorphous, they are stored using cryoprotective agents such as
glycerine and dimethyl sulfoxide (Me2SO). The speed of ice crystal
formation is thus further restricted during preservation below a
certain temperature, and complete vitrification is possible. -130°C is
the recrystallization point of pure water in the ultra-low temperature
zone. This is the temperature at which amorphous ice crystals
recrystallize. For a mixed solution containing Me2SO and other
cryoprotectants, recent research confirms that recrystallization occurs
around -115°C. Thus samples maintained in an ultra-low temperature
freezer at -150°C, far lower than the recrystallization point, can be
semipermanently preserved. Such preservation maintains vitrification
without further crystallization within and outside cells. Other recent
findings show that preserving cattle sperm at -135°C is insufficient,
and also that superconductivity experiments require temperatures of at
least -148°C. These cases show the increased necessity of -150°C
freezing in a diverse array of cryogenic applications.
Applications
- Preservation
- Cancer Research: Tumor Cell Preservation
- Blood or Bone Marrow Preservation
- Bacteria Research: Virus Preservation
- Sperm and Fertilized Ovum (Bull, Goat, Horse, Pig, Chicken)
Preservation
- Pollen and Plant Cell Preservation
- Monoclonal Antibody Preservation
- Environmental Experiment
- Superconductivity Research
- Electronics Experimentation (-148°C)
- Typical Installations
- Universities and Private Research Institutes
- Public Research Center
- Hospitals
Advanced Features
- Application Specific Ultra Low Temperature Compressor
- Panasonic patented VIP® Plus Vacuum Insulation Panel for high density
- Three levels of cabinet security
- High performance, quiet-running Panasonic-designed refrigeration
The Ideal -150°C, Freezing Environment in 8.2 cu.ft.
- LN2 Cost Savings
- Safe and Convenient Usability
- Innovative Control with LCD panel
- Reduces Cell Cross Contamination
- State Storage Temperatures
- Increased Cell Viability - Long Term Preservation
Ideal Alternative to LN2 Storage
Compared to liquid nitrogen systems, Panasonic MDF-C2156VANC offers the following advantages:
- Lower running cost
- Easy maintenance
- No occurrence of contamination
Mechanical Freezer Preservation
Freezer preservation provides users with numerous advantages;
provides uniform cryogenic storage temperatures; no worries about sample
contamination, no liquid supply problems, no danger of sudden liquid
eruptions, and low operational costs.
This freezer provides easier and more stable long-term storage below the recrystallization point than ever before.
An Ideal Freezing Environment By Means VIP® PLUS Design
In Ultra-Low temperature freezers, effective insulation is extremely
critical since the temperature difference between the outside and the
inner compartment can be as high as 115°C.
Panasonic Safety Designs
Until recently, liquid nitrogen preservation containers were mainly
used when preserving valuable samples over long periods. This method,
however, involved troublesome liquid control and the dangers of a liquid
supply. In addition, mycoplasma and other organisms can cause cell and
tissue contamination in liquid phase preservation. As a solution to this
problem, demand for vapor phase preservation has increased. In
preservation with liquid nitrogen vapor, temperatures drop to
approximately -150°C, almost the same as the Panasonic cryogenic freezer
inner cabinet temperature of - 152 degrees C.
Preservation in the Panasonic mechanically refrigerated cryogenic freezer provides users with numerous advantages including:
- no worries about sample contamination,
- no liquid supply problems,
- no danger of sudden liquid eruptions,
- and low operational costs.
Panasonic cryogenic freezers provide easier and more stable long-term storage below the recrystallization point than ever before.
Refrigeration System Features
- Panasonic has developed a special mixed refrigerant, replacing
chlorofluorocarbons (CFCs) that deplete the ozone layer. This new
cooling system virtually eliminates damage to the earth's environment.
- Specially designed compressor and cascade refrigeration system for an ultra-low temperatures of -150°C
- A highly efficient, exclusive compressor has been specially developed and incorporated in the freezing unit.
- A refrigeration circuit with Panasonic's original cascade refrigeration system enhances reliability of long-term preservation.
- With a powerful low-noise design afforded by traditional
ultra-low temperature technologies, the Panasonic cryogenic freezer delivers
durable, stable cooling.
Advanced Control Features
- Microprocessor temperature control with LED digital display allows accurate temperature control
- Accurate temperature setting, confirmation and operation are
managed through microprocessor temperature control with a LED digital
display and flat key data entry.
- This model maintains inner cabinet temperature safely below the -130°C recrystallization point for pure water:
- Model MDF-2156, -150°C
- This low temperature provides an ideal preservation environment for long-term storage.
Oil Separator
- A high-efficiency oil separator creates stable ultra-low temperature environments.
- Compressors continuously repeat highly compressed operations, so
lubricant oil is essential to prevent abrasion and seizure. But when
lubricant oil circulates in the refrigeration circuit, piping becomes
clogged and results in compressor damage.
- Incorporating an exclusive high-efficiency oil separator, the Panasonic cryogenic freezer effectively separates lubricant oil from the
refrigerant, offering a stable ultra-low temperature environment.
Cabinet Insulation
A special foamed-in-place polyurethane insulation material offers
maximum cabinet protection for operating efficiency and stored product
safety.
- The temperature difference between the inside and outside of the freezer can reach a maximum of 150°C Model MDF-1156).
- In the cryogenic range below - 100°C, ordinary foamed-in-place polyurethane insulating material can become cracked and warped.
- Specially designed to withstand low temperatures, Panasonic
foamed-in-place polyurethane insulation is 6.7"" (170mm) thick and
highly resistant to extreme temperature differences, thus helping
maintain inner temperature stability.
VIP® PLUS Design
- Panasonic VIP® PLUS Cryogenic Series -150°C ultra-low temperature freezers use patented revolutionary vacuum insulation cabinet construction
that reduces the wall thickness from 175mm ti 135mm (6.9"" to 5.3"") and
achieves up to 25% more storage capacity than a conventionally
insulated freezer without increasing the footprint.
- This design prevents the insulation layers from distortion and
cracking that might occur due to temperature differences inside and out,
and creates the most efficient insulation material available today.
- Moisture condensation at the top edges of the cabinet due to
differences in temperature inside and out causes frost and icing
problems, which may reduce heat insulation efficiency and obstruct door
movements. These obstructions are prevented by the "hot line" which
prevents frost and condensation from forming on the gasket.
- The newly developed refrigeration system and the freezer structure offer a quiet experimental environment.
- Achieves 50% higher energy conservation than conventionally insulated model.
Control, Alarm and Monitoring
Multiple alarm and safety devices are included to protect valuable samples.
- Microprocessor-controlled filter-clogged check function protects the refrigeration circuit.
- High temperature warning equipment automatically indicates when the temperature deviates 10°C from the set temperature.
- The power failure alarm lamp and buzzer are activated in case of power failure or irregular temperature increase.
- A remote alarm contact is equipped.
- The unit is also equipped with an auxiliary back-up system for
liquid nitrogen that works for approximate 8-12 consecutive hours.
Cabinet Design
The inner cabinet configuration is easy to use and accommodates standard 2"" and 3"" high boxes in vertical racks.
All cabinets include
- 1 set of keys,
- 2 rubber caps,
- 1 inside lid,
- 1 defrost spatula,
- 1 mat (SUS-304),
- connecting pipe for back-up system,
- 1 battery
Specially Designed Compressor for Cryogenic Temperatures The
refrigeration system is at the heart of producing cryogenic temperature
freezing. In the process of developing various technical solutions in
order to achieve and maintain cryogenic temperatures, Panasonic designed
compressor specifically for this use. Panasonic is a leading manufacturer of
cryogenic temperature freezers that develops and produces all its own
components, from the refrigeration units to the micro-chips.