-150°C Cryogenic Temperature Freezer Ensures Stable Cell and Tissue preservation
Cryopreservation allows for storage of biological materials at ultra
low temperatures, while minimizing the level of freezing damage to
cells. An important factor to consider when preserving cells or tissue
at cryogenic temperatures is to prevent amorphous ice crystals from
recrystallizing within and outside the cells. These ice crystals
increase the degree of freezing damage to cells. When ice crystals
become amorphous, they are stored using cryoprotective agents such as
glycerine and dimethyl sulfoxide (Me2SO). The speed of ice crystal
formation is thus further restricted during preservation below a
certain temperature, and complete vitrification is possible. -130°C is
the recrystallization point of pure water in the ultra-low temperature
zone. This is the temperature at which amorphous ice crystals
recrystallize. For a mixed solution containing Me2SO and other
cryoprotectants, recent research confirms that recrystallization occurs
around -115°C. Thus samples maintained in an ultra-low temperature
freezer at -150°C, far lower than the recrystallization point, can be
semipermanently preserved. Such preservation maintains vitrification
without further crystallization within and outside cells. Other recent
findings show that preserving cattle sperm at -135°C is insufficient,
and also that superconductivity experiments require temperatures of at
least -148°C. These cases show the increased necessity of -150°C
freezing in a diverse array of cryogenic applications.
Compared to liquid nitrogen systems, Panasonic MDF-C2156VANC offers the following advantages:
Freezer preservation provides users with numerous advantages;
provides uniform cryogenic storage temperatures; no worries about sample
contamination, no liquid supply problems, no danger of sudden liquid
eruptions, and low operational costs.
This freezer provides easier and more stable long-term storage below the recrystallization point than ever before.
In Ultra-Low temperature freezers, effective insulation is extremely
critical since the temperature difference between the outside and the
inner compartment can be as high as 115°C.
Until recently, liquid nitrogen preservation containers were mainly
used when preserving valuable samples over long periods. This method,
however, involved troublesome liquid control and the dangers of a liquid
supply. In addition, mycoplasma and other organisms can cause cell and
tissue contamination in liquid phase preservation. As a solution to this
problem, demand for vapor phase preservation has increased. In
preservation with liquid nitrogen vapor, temperatures drop to
approximately -150°C, almost the same as the Panasonic cryogenic freezer
inner cabinet temperature of - 152 degrees C.
Preservation in the Panasonic mechanically refrigerated cryogenic freezer provides users with numerous advantages including:
Panasonic cryogenic freezers provide easier and more stable long-term storage below the recrystallization point than ever before.
A special foamed-in-place polyurethane insulation material offers
maximum cabinet protection for operating efficiency and stored product
Multiple alarm and safety devices are included to protect valuable samples.
The inner cabinet configuration is easy to use and accommodates standard 2"" and 3"" high boxes in vertical racks.
Specially Designed Compressor for Cryogenic Temperatures The
refrigeration system is at the heart of producing cryogenic temperature
freezing. In the process of developing various technical solutions in
order to achieve and maintain cryogenic temperatures, Panasonic designed
compressor specifically for this use. Panasonic is a leading manufacturer of
cryogenic temperature freezers that develops and produces all its own
components, from the refrigeration units to the micro-chips.